experimental study on the pathogenicity of avian influenza a/ch/it/5093/99(h7n1) virus in chicken
Authors
abstract
in this study, the pathogenicity of a/ch/it/5093/1999 h7n1 which had been isolated from chicken during the outbreak in italy was assessed in chicken by experimental infection virus. ten spf chickens of four week-old were inoculated with this virus, and five chickens were inoculated with uninfected allantoic fluid. for determination of virus shedding, oropharyngeal and cloacal swabs were taken from experiment and control groups, on days 1-5 post infection (p.i.) and used in a reverse transcription-polymerase chain reaction (rt- pcr) assay for detection of avian influenza virus (aiv). on day (2-5 d.p.i.) certain organs such as lung, brain, liver and kidneys collected from dead birds for virus titration, and histopathological investigations. in this study we obtained high titers in oropharyngeal swabs in 48 hours p.i. (h.p.i.). the first clinical signs observed were anorexia and depression in chickens. the results obtained with the virus isolation were confirmed by rt-pcr. all of the chickens inoculated with a/ch/it/5093/99 died between 2d.p.i and 5 d.p.i. (2-5 d.p.i). on day 1 to 3 d.p.i. relatively high titer of infectious virus could also be isolated from oropharyngeal swab 102.3 to105.9 50% egg infectious doses (eid50/ml), whereas virus shedding from the cloaca (101.5 to 103.2 eid50/ml) was considerably less. the present study was conducted to determine the pathogenicity of a/ch/it/5093 h7n1 in chickens. in our data show that infection of chicken with h7n1 virus leads to viral replication on the respiratory tract resulting in severe lung damage. the most consistent and severely affected organ by this virus were lung, kidney and brain, severity of the lesions in each organ was probably related to tissue tropism.
similar resources
Experimental Study on the Pathogenicity of Avian Influenza A/Ch/It/5093/99(H7N1) Virus in Chicken
In this study, the pathogenicity of A/Ch/It/5093/1999 H7N1 which had been isolated from chicken during the outbreak in Italy was assessed in chicken by experimental infection virus. Ten SPF chickens of four week-old were inoculated with this virus, and five chickens were inoculated with uninfected allantoic fluid. For determination of virus shedding, oropharyngeal and cloacal swabs were taken f...
full textA Study on Histopathologic Changes in Chicken Following Intravenous Inoculation with Avian Influenza Virus A/Chicken/Iran/259/1998(H9N2)
full text
Evaluation of the Pathogenicity of A/Chicken/Iran/ZMT-173/99 H9N2 Strain of Avian Influenza Virus in Serologically Mycoplasma Galisepticum Positive and Negative Broiler Chickens
full text
Thermal inactivation of H5N1 high pathogenicity avian influenza virus in naturally infected chicken meat.
Thermal inactivation of the H5N1 high pathogenicity avian influenza (HPAI) virus strain A/chicken/Korea/ES/2003 (Korea/03) was quantitatively measured in thigh and breast meat harvested from infected chickens. The Korea/03 titers were recorded as the mean embryo infectious dose (EID50) and were 10(8.0) EID50/g in uncooked thigh samples and 10(7.5) EID50/g in uncooked breast samples. Survival cu...
full textExperimental study to determine if low-pathogenicity and high-pathogenicity avian influenza viruses can be present in chicken breast and thigh meat following intranasal virus inoculation.
Two low-pathogenicity (LP) and two high-pathogenicity (HP) avian influenza (AI) viruses were inoculated into chickens by the intranasal route to determine the presence of the AI virus in breast and thigh meat as well as any potential role that meat could fill as a transmission vehicle. The LPAI viruses caused localized virus infections in respiratory and gastrointestinal (GI) tracts. Virus was ...
full textA model for emergence of high pathogenicity avian influenza virus from outbreaks with low pathogenicity avian influenza virus
In this article, we establish a mathematical model for emergence of high pathogenicity avian influenza (HPAI) viruses from outbreaks of low pathogenicity avian influenza (LPAI) A viruses (H5N2). The model is based on traditional mathematical epidemiology models and experiment evidences. It contains several submodels which are traditional SEIR models or SIR models. The experiments in [25] provid...
full textMy Resources
Save resource for easier access later
Journal title:
archives of razi institutePublisher: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
volume 63
issue 1 2016
Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023